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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
29/11/2022 |
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Data da última atualização: |
29/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAVALCANTE, M. de A.; OLIVEIRA, J. dos S.; BARRETO, M. S. da S.; PINHEIRO, L. P.; CANTUÁRIA, P. de C.; BORGES, W. L.; SILVA, G. A. da; SOUZA, T. M. de. |
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Afiliação: |
MARÍLIA DE A. CAVALCANTE, INSTITUTO FEDERAL DO AMAPÁ; JANYNA DOS S. OLIVEIRA, INSTITUTO FEDERAL DO AMAPÁ; MAYRA S. DA S. BARRETO, UNIVERSIDADE DO ESTADO DO AMAPÁ; LUCAS P. PINHEIRO, UNIVERSIDADE DO ESTADO DO AMAPÁ; PATRICK DE C. CANTUÁRIA, INSTITUTO DE PESQUISAS CIENTÍFICAS E TECNOLÓGICAS DO ESTADO DO AMAPÁ; WARDSSON LUSTRINO BORGES, CPAF-AP; GABRIEL A. DA SILVA, UNIVERSIDADE DO ESTADO DO AMAPÁ; TIAGO MARCOLINO DE SOUZA, UNIVERSIDADE DO ESTADO DO AMAPÁ. |
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Título: |
An HPLC method to determine phenolic compounds of plant extracts: application to Byrsonima crassifolia and Senna alata leaves. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pharmacognosy Research, v. 14, n. 4, p. 395-404, 2022. |
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DOI: |
10.5530/pres.14.4.58 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work. MenosBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the... Mostrar Tudo |
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Palavras-Chave: |
Compostos bioativos; Método cromatográfico. |
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Thesagro: |
Cromatografia; Extrato Vegetal. |
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Thesaurus Nal: |
Bioactive compounds; Plant extracts. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148941/1/CPAF-AP-AnHPLCMethod.pdf
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Marc: |
LEADER 02625naa a2200289 a 4500
001 2148941
005 2022-11-29
008 2022 bl uuuu u00u1 u #d
024 7 $a10.5530/pres.14.4.58$2DOI
100 1 $aCAVALCANTE, M. de A.
245 $aAn HPLC method to determine phenolic compounds of plant extracts$bapplication to Byrsonima crassifolia and Senna alata leaves.$h[electronic resource]
260 $c2022
520 $aBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work.
650 $aBioactive compounds
650 $aPlant extracts
650 $aCromatografia
650 $aExtrato Vegetal
653 $aCompostos bioativos
653 $aMétodo cromatográfico
700 1 $aOLIVEIRA, J. dos S.
700 1 $aBARRETO, M. S. da S.
700 1 $aPINHEIRO, L. P.
700 1 $aCANTUÁRIA, P. de C.
700 1 $aBORGES, W. L.
700 1 $aSILVA, G. A. da
700 1 $aSOUZA, T. M. de
773 $tPharmacognosy Research$gv. 14, n. 4, p. 395-404, 2022.
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Registro original: |
Embrapa Amapá (CPAF-AP) |
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| Registros recuperados : 275 | |
| 83. |  | COTA, L. V.; SILVA, D. D. da; COSTA, R. V. da; LANZA, F. E.; AGUIAR, F. M. Ameaça branca. Cultivar Grandes Culturas, Pelotas, v. 17, n. 201, p. 6-7, fev. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 85. | | SILVA, D. D. da; CASELA, C. R.; MACIEL, C. T.; FREITAS, M. E.; COSTA, R. V. da. Associação de virulência em linhagens Elites de sorgo a nove raças de Peronosclerospora sorghi e previsão de resistência de seus híbridos. In: CONGRESSO NACIONAL DE MILHO E SORGO, 27.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 3.; WORKSHOP SOBRE MANEJO E ETIOLOGIA DA MANCHA BRANCA DO MILHO, 2008, Londrina. Agroenergia, produção de alimentos e mudanças climáticas: desafios para milho e sorgo: trabalhos e palestras. [Londrina]: IAPAR; [Sete Lagoas]: Embrapa Milho e Sorgo, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 87. | | PARREIRA, D. F.; ZAMBOLIM, L.; NEVES, W. dos S.; COSTA, R. V. da; COTA, L. V.; SILVA, D. D. da. A antracnose do milho. Revista Trópica - Ciências Agrárias e Biológicas, Chapadinha, v. 8, n. 1, p. 11-27, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 88. | | NOLASCO, A. A. R.; COSTA, R. V. da; COTA, L. V.; FERREIRA, P.; SILVA, D. D. da; PARREIRA, D. F. Avaliação de cultivares de milho quanto à incidência de grãos ardidos. In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 93. | | SILVA, D. D. da; COTA, L. V.; MAY, A.; COSTA, R. V. da; PARREIRA, D. F.; LANZA, F. E. Controle químico da helmintosporiose em sorgo sacarino. In: CONGRESSO NACIONAL DE MILHO E SORGO, 30.; SIMPÓSIO SOBRE LEPDÓPTEROS COMUNS A MILHO, SOJA E ALGODÃO, 1., 2014, Salvador. Eficiência nas cadeias produtivas e o abastecimento global: resumos expandidos. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2014. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 96. | | PACHECO, R. C.; SILVA, D. D. da; MENDES, S. M.; LIMA, K. P.; FIGUEIREDO, J. E. F.; MARUCCI, E, C. How omnivory affects the survival and choices of earwig Doru luteipes (Scudder) (Dermaptera: Forficulidae)? Brazilian Journal of Biology, v. 83, e243890, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 275 | |
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